Several laboratories worldwide neurotoksinitas affects enormous challenges in screening chemicals for growing catalog which identify the risks to human health, with the introduction of legislation mnegacu - invitation of the scope of the EU. The in vitro toxicity testing has traditionally been done using animals, but with the excess of expenditure relating to the funds and the number of animals, salinity reliable penggujian necessary. The lack of comparable readings between in vivo and in vitro systems can mnyajikan suaatu problems, said Jonathan West at the University of Dortmund, Germany, but West said he had to solve these problems by using neural networks.
This new test platform, called the test networking (NFA), to monitor the connection (or changes), especially between neurons - the neurons are placed and their neighbors. The formation of some of these connections is one of the basic principles in memory and learning, and is often the clinical signs neurotoksinitas gangguannnya, West said. "Because the NFA test the connections of neurons following is an in vitro model is comparable to the in vivo situation," he added.
West to neurons - in the composition of human neurons segilima using patterns of cells - and then, easy to control the formation of networks. Composition becomes acrylamida exposure, a neurotoxin that blocks the reference compounds and networking to distinguish between the effects of neurotoxins with cytiotoksin. Without the need to place neurons - neurons or measure the length of connections, this means that a typical arrangement should only take three hours, compared with more than 200 hours of manual testing.
"Application of micro system simple and sophisticated - this engineering can significantly improve the biological standard protocol," says Marco Cecchini, an expert on high-level bio pemolaan resolution, the National Enterprise for Nanoscience and Nanotechnology, Pisa, Italy.
"NFA also apply in the context of reproductive testing of toxin levels are expected mngkonsumsi approximately 60% of all animals requires outreach and other areas necessary for the new assay in vitro 'addition of Marcel Leist University of Konstanz, Germany, who worked on this project with the West.
Today, the team is working with teams across Europe to adopt the NFA in use with a network of neural cells, but 'real litmus test digunakankah NFA or not taken by the laboratory neurotoksikologi and accepted by the authorities, "said West.
This new test platform, called the test networking (NFA), to monitor the connection (or changes), especially between neurons - the neurons are placed and their neighbors. The formation of some of these connections is one of the basic principles in memory and learning, and is often the clinical signs neurotoksinitas gangguannnya, West said. "Because the NFA test the connections of neurons following is an in vitro model is comparable to the in vivo situation," he added.
West to neurons - in the composition of human neurons segilima using patterns of cells - and then, easy to control the formation of networks. Composition becomes acrylamida exposure, a neurotoxin that blocks the reference compounds and networking to distinguish between the effects of neurotoxins with cytiotoksin. Without the need to place neurons - neurons or measure the length of connections, this means that a typical arrangement should only take three hours, compared with more than 200 hours of manual testing.
"Application of micro system simple and sophisticated - this engineering can significantly improve the biological standard protocol," says Marco Cecchini, an expert on high-level bio pemolaan resolution, the National Enterprise for Nanoscience and Nanotechnology, Pisa, Italy.
"NFA also apply in the context of reproductive testing of toxin levels are expected mngkonsumsi approximately 60% of all animals requires outreach and other areas necessary for the new assay in vitro 'addition of Marcel Leist University of Konstanz, Germany, who worked on this project with the West.
Today, the team is working with teams across Europe to adopt the NFA in use with a network of neural cells, but 'real litmus test digunakankah NFA or not taken by the laboratory neurotoksikologi and accepted by the authorities, "said West.
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